Clinical Studies—Why They’re the Gold Standard for Supplements

Since launching AG1 in 2010, we’ve continuously developed the product based on the latest scientific insights. Now, we’re taking the next step—with four new randomised controlled trials (RCTs) on AG1 and a clear commitment to further invest in clinical research in the years to come. Our goal: to raise the standard in the supplement industry.

Why Do Clinical Studies Matter?

Countless scientific studies on nutrients, bacteria and holistic health form the foundation of modern supplement development. But theoretical evidence alone isn’t enough—what’s needed are real-world data on real people. That’s our standard.

We believe benefits shouldn’t just be felt—they should be measurable. In an increasingly saturated market, clinical results are key to understanding what actually works. They show how a product performs in practice—not just on paper.

What Are Clinical Studies?

Clinical studies are structured investigations designed to test how a product works, its effectiveness, safety and tolerability. They’re conducted on human volunteers and follow strict scientific and ethical protocols, such as the internationally recognised Good Clinical Practice (GCP) guidelines.

The aim is to generate reliable, meaningful results under clearly defined conditions—based on sound, verifiable data.

Good to know: while clinical studies are legally required for pharmaceuticals, they remain voluntary—and still uncommon—for supplements.

What Kind of Studies Has AG1 Conducted?

In addition to preclinical and observational studies, we work with academic partners and independent research institutions to run randomised, placebo-controlled trials (RCTs). Why?

What makes an RCT so robust?

• Randomisation: Participants are randomly assigned to the test or control group—minimising bias.
• Control groups: Results are compared against a placebo or standard treatment.
• Blinding: Neither participants nor researchers know who receives what—eliminating expectation bias.
• Predefined endpoints: The impact of the product is measured against clear and consistent criteria.
• Statistical rigour: Ensures results are not just chance—but statistically significant.

Why Clinical Studies Are Still the Exception

Clinical research is still rare in the supplement industry—for a reason. It requires time, resources, expertise and strict methodological standards. But that’s exactly why it matters. Anyone committed to scientific rigour is also committing to transparency, validation and continuous improvement.

Our Clinical Trials at a Glance

The AG1 Upgrade was studied in four independent randomised controlled trials—all using gold-standard methodology. Key results showed rapid nutrient absorption and a tenfold increase in beneficial gut bacteria.

Here’s a breakdown of the studies:

• A 12-week, parallel-group RCT with 105 adults
  Participants followed a standard American diet. This long-term study tested how daily AG1 use affected nutrient status compared to placebo. Researchers also examined gut microbiome changes, safety and tolerability.
• A 2-week crossover RCT with 20 highly active adults
  In crossover trials, participants receive both placebo and active treatment at different points—allowing for precise within-group comparisons. This study explored nutrient status, gut microbiome impact and changes after stopping AG1. It also measured tolerability and safety.
• A 2-week crossover RCT with 24 healthy adults with occasional digestive issues
  The study focused on changes to the gut microbiome in individuals with mild digestive complaints, including any shifts after discontinuing AG1. It also measured tolerability and nutrient status.
• An acute crossover RCT with 16 healthy adults
  This short-term study assessed the bioavailability of key nutrients over an 8-hour period after a single serving of AG1. Secondary goals included overall nutrient status and immediate tolerability.

Want to learn more about our clinical results? This way.

Also Worth Knowing—In Vitro Studies

Alongside our clinical trials, we’ve built a strong foundation of preclinical research—including in vitro studies designed to better understand how AG1 works.

We’ve partnered with ProDigest, a global leader in gut modelling, to explore the effects of AG1 using the SHIME® system (Simulator of the Human Intestinal Microbial Ecosystem).

SHIME is the industry-leading method for simulating human digestion. It mimics the stomach, small intestine and various sections of the colon using a series of connected bioreactors—allowing us to observe how AG1 behaves in the gut, without invasive procedures on humans.

SHIME is one of the only models that recreates the full digestive tract, making it a powerful tool for studying how ingredients survive and function in different parts of the gut.

Glossary at a Glance

Here’s a quick reference for the most important ones—clearly explained.