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    Our daily core supplement blend.

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  1. Home
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  4. Why Third-Party Testing, Independent Certifications, and ISO Standards Matter

Why Third-Party Testing, Independent Certifications, and ISO Standards Matter

Anyone can make bold claims—but not every brand proves them. At AG1, we go beyond industry norms with independent testing, certified labs, certificates of analysis and full transparency.

Written by AG1 Editorial Team
5 MIN READ — 06/24/2025

At AG1, quality is a system built on science, transparency, and accountability at every step: From ingredient sourcing to formulation, manufacturing to post-production testing, every batch of AG1 is held to a rigorous set of standards that exceed the norm.

In this article, we’re pulling back the curtain. What does third-party testing actually involve? Why do certifications like Kölner Liste® and Informed Choice matter? What’s an ISO-certified lab? And how can you tell if a product is truly clean and reliable? Let’s dive in.

What We'll Cover

  • 01.

    First: What Do We Mean by ‘Quality’ in the Supplement World?

  • 02.

    The Limits of Regulation and the Role of Third-Party Testing

  • 03.

    What Do Labs Actually Test For?

  • 04.

    What About Certifications? Why They Matter (and Why AG1 Has Them)

  • 05.

    The Certificate of Analysis: Your Window Into What’s Inside

  • 06.

    Why All This Matters (and Why So Few Brands Do It)

Here’s what you get with AG1:

  • 01.

    Independent third-party testing across all batches

  • 02.

    Certified by both Informed Choice and Cologne List®

  • 03.

    Tested in ISO-certified labs

  • 04.

    Publicly available batch-specific Certificates of Analysis

  • 05.

    Constant formula innovation based on science and testing

First: What Do We Mean by ‘Quality’ in the Supplement World?

Quality in supplements is about more than just having ‘the right’ ingredients. At AG1, we define quality as:

  • Identity: Each ingredient must be what we say it is. No substitutions, no fillers.
  • Purity: Every batch must be tested for contaminants like heavy metals, pesticides, or microbes.
  • Potency: The amount of each ingredient must match the label claim.
  • Consistency: Every batch needs to meet the same high standards.
  • Transparency: You have the right to know what’s in your product, and how we tested it.

While this sounds obvious, many supplements on the market fall short in one or more of these areas. That’s why robust quality control processes are essential—especially when you're consuming a product daily.

The Limits of Regulation and the Role of Third-Party Testing

Unlike pharmaceutical drugs, dietary supplements in both the EU and the UK do not require pre-approval before hitting the market. That means the responsibility for safety, quality, and labelling falls on the manufacturer—and not every company takes this seriously.

Government bodies such as the EFSA (European Food Safety Authority) and FSA (Food Standards Agency) set rules around food supplements, but they rarely inspect or test products proactively. Instead, they act retroactively—stepping in only if a problem is reported.

That’s where third-party testing plays a crucial role. It acts as an external quality check, filling the regulatory gap and ensuring that claims are backed up by real data.

What Is Third-Party Testing?

Third-party testing means that an ISO-certified independent laboratory—not owned by the supplement brand—carries out a range of scientific analyses on the product. This ensures:

- Competence: Reliable personnel, facilities and equipment.
- Testing Methods: Use of valid and appropriate methods for testing.
- Quality Assurance: Accuracy and reliability of test results.

Think of it as the nutritional world’s version of scientific peer review.

All of AG1’s third-party testing is conducted in ISO/IEC 17025/215 accredited laboratories. That number might look technical—and it is—but it’s the gold standard for lab competence.

Here’s why it matters:

  • Labs are audited by independent accreditation bodies
  • Testing methods must be scientifically validated and documented
  • Results must meet strict standards for accuracy, repeatability, and traceability

In other words, ISO certification ensures that labs aren’t just doing tests—they’re doing them right.

What Do Labs Actually Test For?

Let’s break down the core areas third-party labs evaluate:

1. Ingredient Identity

This confirms that each listed ingredient is authentic and correctly identified. Methods like high-performance liquid chromatography (HPLC), mass spectrometry, or DNA barcoding (for botanicals) are used to verify identity—and rule out adulteration or mislabelling.

2. Purity

Here, labs check for unwanted substances such as:

  • Heavy metals like lead, mercury, or cadmium
  • Pesticide residues from plant-based ingredients
  • Microbial contamination (bacteria, mould, or yeasts)
  • Residual solvents from ingredient extraction processes
  • Undeclared allergens or pharmaceuticals

These can affect not only efficacy, but also safety.

3. Potency

This assesses whether the amount of each nutrient matches what the label claims. Inconsistent or sub-potent formulas are unfortunately common in the supplement world.

But potency isn’t just about truth in labelling. It’s also a legal and functional requirement for making health claims. In the EU and UK, you can only communicate the health benefits of a nutrient if it's present at a level high enough to meet official thresholds set by regulators. For example, you can’t claim that a product “contributes to normal energy metabolism” via vitamin B12 unless the formula contains a sufficient, bioavailable dose of that nutrient.

This is why robust potency testing matters. It ensures not only that you get what you're paying for—but that any claims made on the product are both scientifically valid and legally compliant.

4. Stability and Shelf-Life

While not always included in third-party testing, some brands (like AG1) go further to confirm that key nutrients remain stable over time. Because a daily supplement should deliver benefits not just today, but six months from now too.

Third-party testing acts as an external quality check, filling the regulatory gap and ensuring that claims are backed up by real data.

What About Certifications? Why They Matter (and Why AG1 Has Them)

Certifications are independent stamps of approval from recognised third-party organisations.

Cologne List®

This is one of Europe’s most trusted databases for doping-free sports nutrition. Products listed here are tested for substances banned in professional sport—making it a go-to resource for athletes and informed consumers alike.

AG1 is listed on the Cologne List®, confirming that our product is free of substances prohibited by WADA (World Anti-Doping Agency) and safe for competitive use.

Informed Choice

Operated by the globally respected LGC group, this certification is widely recognised in elite sport. It involves:

  • Regular product testing for over 250 banned substances
  • Audits of manufacturing facilities
  • Ongoing batch testing to ensure compliance
AG1 proudly holds Informed Choice certification, offering peace of mind for everyone from Olympic athletes to anyone who is serious about their wellness.

The Certificate of Analysis: Your Window Into What’s Inside

Every batch of AG1 is accompanied by a Certificate of Analysis (CoA)—a quality assurance document that summarises the test results from our third-party lab partners and verifies the formula's composition and confirms the absence of contaminants.

A CoA typically includes:

  • Ingredient identity and strength results
  • Microbiological testing results
  • Heavy metal and pesticide screening
  • Pass/fail status for each quality marker

We publish these certificates here to provide transparent insights into our quality testing.

Why All This Matters (and Why So Few Brands Do It)

The supplement industry is crowded. But very few brands go to the lengths we do to guarantee quality, and even fewer are willing to be this transparent.

You shouldn’t have to be a scientist to feel confident about what you put in your body. But if you are curious—we’re ready to show you the science.

Your trust is everything to us. And we’ve built our quality systems to honour that. Find out more about our quality standards here.

Who Wrote This

AG1 Editorial Team

Our in-house editorial team

The AG1 Editorial Team is our in-house group of writers and editors. Working closely with AG1's nutrition and research teams, they turn current science into clear, practical articles, and every piece is reviewed by a qualified nutrition expert before it is published.

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Food supplements are not a substitute for a balanced and varied diet and a healthy lifestyle. Do not exceed the indicated daily intake. Keep out of reach of children. Please inform and consult your doctor before consuming this product. Not suitable for children and adolescents under 18 years, pregnant or breastfeeding women. Refrain from taking other food supplements containing zinc. Persons taking anticoagulant medication should seek medical advice before consuming vitamin K-containing supplements.

* Genuine reviews from customers who received a review link after purchase.

**Offer valid for new customers only when signing up for a 3-month AG1 subscription with upfront payment. Regular price €227. Welcome Kit and gifts are one-time only. Subscription renews automatically unless cancelled beforehand.

↡ Ongoing survey of 3,000+ AG1 customers after 12+ days of use.

§ In a third-party, observational study conducted on 35 healthy adults ages 25-48 assessing the self-perceived efficacy of AG1 over the course of 3 months. The study was conducted on a similar version of AG1's continually evolving formulation. The study results are transferable because of comparable ingredients and amounts.

○ Ongoing survey of customers from 12 European countries who have been taking AG1 for an average of 9 days.

⬥ AG1 single subscriptions price and dose-matched average aggregated prices in 9 individual nutritional supplement categories with comparable ingredients. The prices of the individual supplements are the average prices of three individual products available online for each compared category, each with comparable ingredients. To ensure a fair basis for comparison, where the comparison products have different dosages, the prices were mathematically adjusted to correspond to the dosage contained in AG1. The 9 compared categories are vitamin B12, folate (Methylfolate), vitamin C, vitamin E, bacterial cultures, zinc, coenzyme Q10, greens powder and fibre.

Research Studies
a. In a triple-blind, randomised, placebo-controlled parallel-designed clinical trial evaluating nutrient biomarkers and microbiome shifts in 105 healthy adults ages 20-59 over the course of 12 weeks.
b. In a double-blind, randomised, placebo-controlled 2-week cross-over clinical trial assessing microbiome shifts in 20 active adults ages 19-37.
c. In a double-blind, randomised, placebo-controlled 2-week cross-over clinical trial assessing microbiome shifts of 24 healthy adults with occasional GI distress ages 26-59.
d. In a double-blind, randomised, placebo-controlled cross-over clinical trial assessing bioavailability in 16 healthy adults ages 18-42 over the course of 8 hours.
The studies were conducted on a similar version of AG1's continually evolving formulation. The transferability of the study results is ensured because of comparable ingredients and amounts.

◊ Key health benefits:

(1) Immune system: With vitamins A, C, B6 and B12 as well as folate, zinc, copper and selenium, AG1 contributes to the normal function of the immune system. Vitamin C contributes to maintain the normal function of the immune system during and after intense physical exercise. 200 mg Vitamin C taken daily in addition to the recommended daily dose (80 mg) contributes to the normal function of the immune system during and after intensive physical activity.

(2) Energy levels: Vitamins C, B6, B12, riboflavin, niacin, pantothenic acid and magnesium contribute to normal energy-yielding metabolism and to the reduction of tiredness and fatigue. Magnesium contributes to electrolyte balance. Biotin, zinc and chromium contribute to normal macronutrient metabolism.

(3) Digestion: Calcium contributes to the normal function of digestive enzymes. With vitamin A, biotin, riboflavin and niacin, AG1 contributes to the maintenance of normal mucous membranes (e.g. intestinal mucosa).

(4) Focus; Mental performance; Psychological function; Nervous system: With pantothenic acid, AG1 contributes to normal mental performance. Zinc contributes to normal cognitive function. With vitamins C, B6, B12, B1, B3 and B7 AG1 contributes to normal psychological function and to normal functioning of the nervous system. Calcium contributes to normal neurotransmission.

‡ Additional health benefits:

(5) Muscles: With calcium and magnesium, AG1 contributes to normal muscle function.

(6) Cell Protection: With vitamins E and C as well as riboflavin, zinc, copper and selenium, AG1 contributes to the protection of cells from oxidative stress. Phosphorus contributes to normal function of cell membranes. Folate, Vitamin B6 and B12 contribute to normal homocysteine metabolism.

(7) Skin, hair & nails: With vitamins A, as well as riboflavin, niacin, biotin and zinc, AG1 contributes to the maintenance of normal skin. Vitamin C contributes to normal collagen formation for the normal function of skin. Copper contributes to normal connective tissue. Vitamin B7, zinc and selenium contribute to the maintenance of normal hair. Zinc and selenium contribute to the maintenance of normal nails.

(8) Hormonal system: With vitamin B6, AG1 contributes to the regulation of hormonal activity. Zinc contributes to the maintenance of normal testosterone levels in the blood. Selenium contributes to the normal thyroid function. With chromium, AG1 contributes to the maintenance of normal blood glucose levels.

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