Why Third-Party Testing, Independent Certifications, and ISO Standards Matter

Anyone can make bold claims—but not every brand proves them. At AG1, we go beyond industry norms with independent testing, certified labs, certificates of analysis and full transparency.

Written by AG1 Editorial Team
5 MIN READ — 06/24/2025

At AG1, quality is a system built on science, transparency, and accountability at every step: From ingredient sourcing to formulation, manufacturing to post-production testing, every batch of AG1 is held to a rigorous set of standards that exceed the norm.

In this article, we’re pulling back the curtain. What does third-party testing actually involve? Why do certifications like Kölner Liste® and Informed Choice matter? What’s an ISO-certified lab? And how can you tell if a product is truly clean and reliable? Let’s dive in.

First: What Do We Mean by ‘Quality’ in the Supplement World?

Quality in supplements is about more than just having ‘the right’ ingredients. At AG1, we define quality as:

  • Identity: Each ingredient must be what we say it is. No substitutions, no fillers.
  • Purity: Every batch must be tested for contaminants like heavy metals, pesticides, or microbes.
  • Potency: The amount of each ingredient must match the label claim.
  • Consistency: Every batch needs to meet the same high standards.
  • Transparency: You have the right to know what’s in your product, and how we tested it.

While this sounds obvious, many supplements on the market fall short in one or more of these areas. That’s why robust quality control processes are essential—especially when you're consuming a product daily.

The Limits of Regulation and the Role of Third-Party Testing

Unlike pharmaceutical drugs, dietary supplements in both the EU and the UK do not require pre-approval before hitting the market. That means the responsibility for safety, quality, and labelling falls on the manufacturer—and not every company takes this seriously.

Government bodies such as the EFSA (European Food Safety Authority) and FSA (Food Standards Agency) set rules around food supplements, but they rarely inspect or test products proactively. Instead, they act retroactively—stepping in only if a problem is reported.

That’s where third-party testing plays a crucial role. It acts as an external quality check, filling the regulatory gap and ensuring that claims are backed up by real data.

What Is Third-Party Testing?

Third-party testing means that an independent laboratory—not owned or paid by the supplement brand—carries out a range of scientific analyses on the product. This ensures:

  • Impartiality: The lab has no vested interest in the product passing or failing
  • Standardisation: Methods follow internationally recognised protocols
  • Reproducibility: Results can be replicated and verified

Think of it as the nutritional world’s version of scientific peer review.

What Do Labs Actually Test For?

Let’s break down the core areas third-party labs evaluate:

1. Ingredient Identity

This confirms that each listed ingredient is authentic and correctly identified. Methods like high-performance liquid chromatography (HPLC), mass spectrometry, or DNA barcoding (for botanicals) are used to verify identity—and rule out adulteration or mislabelling.

2. Purity

Here, labs check for unwanted substances such as:

  • Heavy metals like lead, mercury, or cadmium
  • Pesticide residues from plant-based ingredients
  • Microbial contamination (bacteria, mould, or yeasts)
  • Residual solvents from ingredient extraction processes
  • Undeclared allergens or pharmaceuticals

These can affect not only efficacy, but also safety.

3. Potency

This assesses whether the amount of each nutrient matches what the label claims. Inconsistent or sub-potent formulas are unfortunately common in the supplement world.

But potency isn’t just about truth in labelling. It’s also a legal and functional requirement for making health claims. In the EU and UK, you can only communicate the health benefits of a nutrient if it's present at a level high enough to meet official thresholds set by regulators. For example, you can’t claim that a product “contributes to normal energy metabolism” via vitamin B12 unless the formula contains a sufficient, bioavailable dose of that nutrient.

This is why robust potency testing matters. It ensures not only that you get what you're paying for—but that any claims made on the product are both scientifically valid and legally compliant.

4. Stability and Shelf-Life

While not always included in third-party testing, some brands (like AG1) go further to confirm that key nutrients remain stable over time. Because a daily supplement should deliver benefits not just today, but six months from now too.

Third-party testing acts as an external quality check, filling the regulatory gap and ensuring that claims are backed up by real data.

What About Certifications? Why They Matter (and Why AG1 Has Them)

Certifications are independent stamps of approval from recognised third-party organisations. They go beyond lab testing to evaluate overall product safety, quality, and manufacturing practices.

Cologne List®

This is one of Europe’s most trusted databases for doping-free sports nutrition. Products listed here are tested for substances banned in professional sport—making it a go-to resource for athletes and informed consumers alike.

AG1 is listed on the Cologne List®, confirming that our product is free of substances prohibited by WADA (World Anti-Doping Agency) and safe for competitive use.

Informed Choice

Operated by the globally respected LGC group, this certification is widely recognised in elite sport. It involves:

  • Regular product testing for over 250 banned substances
  • Audits of manufacturing facilities
  • Ongoing batch testing to ensure compliance
AG1 proudly holds Informed Choice certification, offering peace of mind for everyone from Olympic athletes to anyone who is serious about their wellness.

ISO-Certified Labs: A Higher Standard of Testing

All of AG1’s third-party testing is conducted in ISO/IEC 17025 accredited laboratories. That number might look technical—and it is—but it’s the gold standard for lab competence.

Here’s why it matters:

  • Labs are audited by independent accreditation bodies
  • Testing methods must be scientifically validated and documented
  • Results must meet strict standards for accuracy, repeatability, and traceability

In other words, ISO certification ensures that labs aren’t just doing tests—they’re doing them right.

The Certificate of Analysis: Your Window Into What’s Inside

Every batch of AG1 is accompanied by a Certificate of Analysis (CoA)—a document issued by our third-party lab partners that verifies the formula's composition and confirms the absence of contaminants.

A CoA typically includes:

  • Ingredient identity and strength results
  • Microbiological testing results
  • Heavy metal and pesticide screening
  • Pass/fail status for each quality marker

We publish these certificates here—because transparency should never be optional.

Why All This Matters (and Why So Few Brands Do It)

The supplement industry is crowded. But very few brands go to the lengths we do to guarantee quality, and even fewer are willing to be this transparent.

You shouldn’t have to be a scientist to feel confident about what you put in your body. But if you are curious—we’re ready to show you the science.

Here’s what you get with AG1:

  • 01.

    Independent third-party testing across all batches

  • 02.

    Certified by both Informed Choice and Cologne List®

  • 03.

    Tested in ISO-certified labs

  • 04.

    Publicly available batch-specific Certificates of Analysis

  • 05.

    Constant formula innovation based on science and testing

Your trust is everything to us. And we’ve built our quality systems to honour that. Find out more about our quality standards here.

Who Wrote This

AG1 Editorial Team

Our internal team of editors

Food supplements are not a substitute for a balanced and varied diet and a healthy lifestyle. Do not exceed the indicated daily intake. Keep out of reach of children. Please inform and consult your doctor before consuming this product. Not suitable for children and adolescents under 18 years, pregnant or breastfeeding women. Refrain from taking other food supplements containing zinc. Persons taking anticoagulant medication should seek medical advice before consuming vitamin K-containing supplements.

* Genuine reviews from customers who received a review link after purchase.

** Offer valid for new subscribers only. Free Shipping does not apply to customers in Switzerland and Norway.

º Food Supplement. In combination with a healthy diet and exercise.

ª Survey on the awareness of multi-nutrient powders with 211 consumers aged 18 to 49 in Germany, 09/24.

↡ Survey of 527 AG1 customers after 12+ days of use, 2024. Vitamins C, B6, B12, riboflavin, niacin, pantothenic acid, and folate help reduce tiredness and fatigue. Pantothenic acid supports mental performance. Calcium supports normal digestive enzyme function, while biotin maintains normal mucous membranes, such as the gut lining.

Immune System

1: With vitamins A, C, B6 and B12 as well as folate, zinc, copper and selenium, AG1 contributes to the normal function of the immune system. Vitamin C contributes to maintain the normal function of the immune system during and after intense physical exercise. The positive effect is achieved if 200 mg of vitamin C are taken daily in addition to the recommended daily dose (80 mg). AG1 contains 420 mg vitamin C.

Metabolism

2: Vitamins C, B6 and B12, as well as thiamine, riboflavin, niacin, biotin, pantothenic acid, calcium, phosphorus, copper, and manganese contribute to normal energy-yielding metabolism. Biotin, zinc, and chromium contribute to normal macronutrient metabolism. Vitamin A and riboflavin contribute to normal metabolism of iron. Zinc contributes to normal acid-base metabolism.

Cell Protection

3: Vitamins E and C, as well as riboflavin, zinc, copper, manganese and selenium, contribute to the protection of cells from oxidative stress. Zinc contributes to normal DNA synthesis. Phosphorus contributes to normal function of cell membranes.

Mental Performance; Mental Focus; Nervous System; Psychological Function

4: Vitamins C, B6, B12, riboflavin, niacin, pantothenic acid and folic acid contribute to the reduction of tiredness and fatigue. Pantothenic acid contributes to normal mental performance. Zinc contributes to normal cognitive function. Vitamins C, B6, B12, thiamine, riboflavin, niacin, biotin, and copper contribute to normal functioning of the nervous system. Thiamine, niacin, vitamin B6, vitamin B12, biotin, folate, and vitamin C in AG1 contribute to normal psychological function.

Bones

5: Calcium contributes to the maintenance of normal bones. Protein, phosphorus, zinc, and manganese contribute to the maintenance of normal bones. Vitamin C contributes to normal collagen formation for the normal function of bones.

Cardiovascular System

6: Thiamine contributes to the normal function of the heart. Vitamins B6 and B12 contribute to normal red blood cell formation. Vitamin C contributes to normal collagen formation for the normal function of blood vessels.

Skin, hair, nails

7: Vitamin A, riboflavin, niacin, biotin and zinc contribute to the maintenance of normal skin. Vitamin C contributes to normal collagen formation for the normal function of skin. Biotin, zinc and selenium contribute to the maintenance of normal hair. Copper contributes to normal skin and hair pigmentation and to normal connective tissue. Selenium and zinc contribute to the maintenance of normal nails.

Hormone system

8: Vitamin B6 contributes to the regulation of hormonal activity. Pantothenic acid contributes to normal synthesis and metabolism of steroid hormones, vitamin D, and some neurotransmitters. Zinc contributes to the maintenance of normal testosterone levels in the blood. Selenium contributes to normal thyroid function and normal spermatogenesis. Zinc contributes to normal fertility and normal reproduction. Chromium contributes to the maintenance of normal blood glucose levels.

Digestion

9: Calcium contributes to the normal function of digestive enzymes. Biotin contributes to the maintenance of normal mucous membranes (e.g., intestinal mucosa).

Muscles

10: Calcium contributes to normal muscle function. Proteins contribute to the maintenance of muscle mass and an increase in muscle mass.

Eyes

11: Vitamin A, riboflavin and zinc contribute to the maintenance of normal vision.